Cells
SPR can be used for drug target measurement, from drug-membrane interaction to drug-cell interaction. Distinguish between interior and penetration in real-time and unlabeled situations. The adhesion of cells on different coatings was studied.
 
There are many drivers behind cell-based studies, including tissue engineering, in vitro toxicology, drug delivery, and antimicrobial coatings. Surface plasmon resonance (SPR) was originally used to measure protein-protein interaction. It was only in recent years that researchers began to develop SPR to study living cells.
 
Some of our users use SPR to measure cell diffusion or adhesion to different biomaterials and coating surfaces.SPR can also be used as a biosensor to detect cell specific metabolites to determine cell differentiation and functionSome groups are studying biofilm formation or antimicrobial coatings
 
Five reasons for choosing SPR to examine living cells:
1. Real time measurement of living cells and bacterial adhesion
2. Able to distinguish infiltration and endocytosis without labeling
3. Nonspecific adsorption on the surface
4. Control of temperature and shear-stress
5. Combination of SPR and electrochemistry
 
Five key questions about living cells that SPR can answer:
1. What is the route of drug absorption?
2. Which kind of nanoparticles is the best carrier for drug delivery?
3. How does a nanoparticle or virus enter the cell?
4. What is the dynamics of cell attachment to the surface?
5. Which surface is the most resistant to bacterial growth?
 
Reference:
1. Endothelial cells' biophysical, biochemical, and chromosomal aberrancies in high ‐ glucose condition within the diabetic range, Cell Biochemistry & Function, 2017, p. 1-15 
2. Feasibility Study of the Permeability and Uptake of Mesoporous Silica Nanoparticles across the Blood-Brain Barrier, PLoS ONE 11(8): e0160705, 2016 
3. Real-Time Label-Free Monitoring of Nanoparticle Cell Uptake, Small, 2016 
4. Real-Time Protein and Cell Binding Measurements on Hydroxyapatite Coatings, J. of Functional Biomaterials, 2016, Vol. 7(3), p. 23 
5. Biomimetic collagen I and IV double layer Langmuir–Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells, Biomaterials, Volume 1, May 2015, Pages 257-269 
6. Structural and Viscoelastic Properties of Layer-by-Layer Extracellular Matrix (ECM) Nanofilms and Their Interactions with Living Cell, ACS Biomater. Sci. Engineering, 2015, 1[9]: p. 816–824 
7. Microbial attachment and adsorption–desorption kinetic of tightly bound extracellular polymeric substances on model organic surfaces, Chemical Engineering Journal, 2015, 279: p. 516–521 
8. Investigation of cell behaviors on thermo-responsive PNIPAM microgel films, Colloids and Surfaces B: Biointerfaces, 132: p. 202–207 
9. Biofunctionalization of titanium surface with multilayer films modified by heparin-VEGF-fibronectin complex to improve endothelial cell proliferation and blood compatibility,  J. of Biomed. Mat. Research Part A, 2013, 101A, 413–420 
10. Oncolytic adenoviruses coated with MHC-I tumor epitopes increase the anti-tumor immunity and efficacy against melanoma, Capasso et al., Taylor & Francis Online, 2015