SPR可(kě)以進行藥物(wù)靶量測量，從藥物(wù)-膜的(de)相互作用(yòng)，到藥物(wù)-細胞的(de)相互作用(yòng)。在實時(shí)和(hé)無标記的(de)情況下(xià)區(qū)分(fēn)内部和(hé)滲透。研究細胞在不同塗層的(de)附著(zhe)情況。
基于細胞的(de)研究有很多(duō)，包括組織工程、體外毒物(wù)學、藥物(wù)輸送，還(hái)有抗菌塗料。表面等離子體共振最初是用(yòng)于蛋白質相互作用(yòng)測量的(de)。直到近年來(lái)，研究人(rén)員(yuán)才開始開發SPR來(lái)研究活細胞。
我們的(de)一些用(yòng)戶使用(yòng)SPR來(lái)測量細胞在不同的(de)生物(wù)材料和(hé)塗層表面的(de)擴散或粘附。
SPR也(yě)可(kě)作爲生物(wù)傳感器用(yòng)于檢測細胞特定代謝物(wù)以确定細胞分(fēn)化(huà)和(hé)細胞功能
一些小組正在研究生物(wù)膜的(de)形成或抗菌塗料
五個(gè)選擇SPR檢查活細胞的(de)原因
1. 能夠實時(shí)測量活細胞和(hé)細菌附著(zhe)
2. 能夠在未标記的(de)情況下(xià)區(qū)分(fēn)滲透和(hé)内吞
3. 表面非特異性吸附情況
4. 溫度和(hé)剪切應力的(de)控制
5. SPR與電化(huà)學的(de)結合
SPR可(kě)以回答(dá)的(de)關于活細胞的(de)五個(gè)關鍵性問題
1. 藥物(wù)吸收途徑是什(shén)麽?
2. 哪一種納米顆粒是藥物(wù)輸送的(de)最佳載體?
3. 一種納米粒子或病毒是如何進入細胞的(de)?
4. 細胞附著(zhe)在表面上的(de)動力學是什(shén)麽?
5. 哪一種表面最能抵抗細菌的(de)生長(cháng)?
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